Figure 2.

PARP inhibition reduces HIV-1 reverse transcriptase (RT) activity and HIV-1 LTR activity in human MDM infected with different tropism HIV-1 strains. RT activity assays performed on HIV-infected MDM treated with PARPi or AZT either for 24 h prior to HIV infection (A,C) or immediately after (B,D) HIV infection. MDM were infected with M-tropic JRFL strain (A,B) or dual-tropic strain 89.6 (C,D). Treatments with PARP inhibitors (PARPi) or AZT were replenished every 24 h. The results are shown as the mean value ± SEM from three independent experiments utilizing MDM prepared from different donors. Data are presented as the percent of HIV-only control. (^*p = 0.05, ^**p = 0.01, ^***p = 0.005, ^****p < 0.0001, ns, not significant).