Figure 6.
PARP inhibition increases inhibitory site Ser3-cofilin phosphorylation. (A) Representative contour plots from flow cytometry analysis of phospho-Ser3-cofilin and total cofilin in MDM stimulated by addition of HIVADA for 5 min. MDM were treated with or without PARPi for 1 h prior to HIVADA introduction. Rho and Rac GTPase inhibitors, CT04, NSC, and Rho/Rac activator, CN04, were used as controls. (B) Quantitation of phosphor-Ser3-cofilin cells in human MDM. The results are shown as the mean value ± SEM (*p < 0.05 vs. HIV-stimulated but inhibitor untreated cells) from three independent experiments utilizing MDM prepared from different donors.