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. 2015 Aug 11;2015:526518. doi: 10.1155/2015/526518

Figure 3.

Figure 3

Expression analysis of SP140L, SP140, SP100, and SP110 in PBMCs and cell lines. (a) The mRNA expression of the SP100 family members in the subpopulations of PBMCs: pDC (plasmacytoid dendritic cells), MO (monocytes), NK (natural killers), B (B cells), and CD4 (CD4+ T cells). (b) The mRNA expression of the SP100 family members in HeLa, HL60, U937, and THP-1 cell lines. (c) The activation of mRNA expression after interferon stimulation in U937 and HL60 cell lines. The mRNA expressions were detected using RT-qPCR. One representative experiment is shown and the error bars show the standard error of the mean (SEM) of the technical replicates. (d) Ectopic expression of SP140L and SP140 proteins in HEK293. The proteins were detected using anti-Myc and anti-FLAG antibodies, respectively.