Table 2.
Primers used in the study.
| Name | Application | 5′ → 3′ |
|---|---|---|
| SP140L_cDNA F1 | cDNA amplification | CAAAGCCGATGGCCGGTGGGGGCA |
| SP140L_cDNA R1 | CAGTAGCATATTTCCTGAAGGCC | |
|
| ||
| SP140L_RACE R1 | 5′ RACE | CAGCTGGCCATCGGCTTTGC |
| SP140L_RACE R2 | TGGTTGTTTTTCTGCGTTCTCG | |
|
| ||
| SP140L F1 | Cloning | TTTGAATTCATGGCAGGTGGGGGCAG |
| SP140L R1 | TTTGTCGACTCAACTGTTCCCATTTGTTTC | |
| SP140L R2 | TTTGCGGCCGCACTGTTCCCATTTGTTTC | |
| SP140L R3 | TTTGGTACCACTGTTCCCATTTG | |
|
| ||
| SP140 F1 | Cloning | TTTGAATTCATGGCCCAGCAGGGCCA |
| SP140 R1 | AAAGTCGACTCAATTGTTCCCATTTGTTTCC | |
| SP140 R2 | TTTGTCGACATTGTTCCCATTTGTTTCCTG | |
| SP140 R3 | TTTGGTACCATTGTTCCCATTTGTTTC | |
|
| ||
| SP110 F1 | Cloning | TTTGAATTCATGTTCACCATGACAAGAGC |
| SP110 R1 | AAAGTCGACTCAAGGAAGAGTCCAGAA | |
| SP110 R2 | TTTGTCGACAGGAAGAGTCCAGAAACC | |
|
| ||
| SP100A F | Cloning | TTTGAATTCATGGCAGGTGGGGGCGGCGA |
| SP100A R | TTTGGTACCATCTTCTTTACCTGACCCTCTTCTTAGG | |
|
| ||
| SP140L_QPCR F1 | Expression analysis | TGGAAGCACTGTTCAGCGAGGT |
| SP140L_QPCR R1 | TGATGTCAGGCCTCTCTTCCCT | |
|
| ||
| SP140_QPCR F1 | Expression analysis | CCAGGTGGGGGAGTGTCCTGT |
| SP140_QPCR R1 | TCTCCCCTGGTGCTGTGCTGT | |
|
| ||
| SP110_QPCR F1 | Expression analysis | CCATACCCCACTGGCGCTGC |
| SP110_QPCR R1 | GTCAGATGGGCTGGGCGAC | |
|
| ||
| SP100_QPCR F1 | Expression analysis | GGCTGAGCCAACAGAGTCCTGCG |
| SP100_QPCR R1 | TCCACCAGTCGCACAGAACAGGA | |
|
| ||
| B2M_QPCR F1 | Expression analysis | TGCTCGCGCTACTCTCTCT |
| B2M_QPCR R1 | TCCATTCTCTGCTGGATGAC | |
|
| ||
| HPRT_QPCR F1 | Expression analysis | GACTTTGCTTTCCTTGGTCAGG |
| HPRT_QPCR R1 | AGTCTGGCTTATATCCAACA CTTCG | |