Figure 2.

Sequence comparison of AP genes in Prorocentrum. (A) Nucleotide substitution of pmnap identified between gDNA and cDNA clones, shaded region represents sharing the same nucleotides and box indicates the nonsynonymous substitution. (B) Schematic of two different genomic fragments of pdoap. (C) Phylogenetic tree inferred from nucleotide sequences (common region) of AP gene in Prorocentrum. Prodo, P. donghaiense; Promini, P. minimum, support nodes shown as NJ/ML. Gradient shaded triangles represent the presence of pseudogenes and the asterisk represents the assembled pdoap ORF from transcriptomic data (intact tree refered to Supplementary Figure 1B).