FIGURE 3.

Effects of TNF on markers of ER stress and zinc metabolism in lean lactating mouse MGs in vivo and differentiated MECs in vitro. Representative immunoblots of protein abundance of HSPA5 in (A) TNF- and vehicle-injected mouse MGs in vivo and (B) HC11 MECs in vitro. (C) Representative immunoblot of protein abundance of ZIP7 in TNF- and vehicle-injected MGs. (D) Zinc concentration of fractions enriched in ER/Golgi apparatus isolated from TNF- and vehicle-injected MGs. Data are means ± SDs, n = 5 mice/group. *P < 0.05. (E) Representative immunoblot of protein abundance of ZIP7 in HC11 MECs in vitro. β-Actin was used as a loading control. ER, endoplasmic reticulum; HSPA5, heat shock protein 5; MEC, mammary epithelial cell; MG, mammary gland; MW, molecular weight; ZIP7, zrt- irt-like protein 7.