FIGURE 4.

1D ¹³C (A) and ¹H (B) NMR spectra of a metabolic mixture. Due to the ¹³C spectral dispersion and narrow peaks, metabolite resonances such as those belonging to isoleucine (peaks indicated by red ticks), can be easily identified. Resonances in the corresponding 1D ¹H spectrum often overlap due to coupling and small spectral width, resulting in difficult analysis. Figure used with permission from Ramaswamy et al. (2013a).