FIGURE 7.

2D INADEQUATE NMR spectrum of the endometabolome of ¹³C-labeled C. elegans. The horizontal axis is the ¹³C chemical shift, and the vertical axis is the double quantum chemical shift. Cross-peaks appear on the double quantum axis at the sum of the two interacting ¹³C frequencies. This spectrum has a very large amount of information, and we developed INETA to extract some of this information in a semi-automated way. Details can be found in the original publication (Clendinen et al., 2015), but some of the output of INETA applied to this spectrum is shown in the dashed lines, which highlight INADEQUATE spin systems from glutamate (brown), lactate (blue), and alanine (green) in the endometabolome.