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. 2015 Aug 25;5:13409. doi: 10.1038/srep13409

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Copyright © 2015, Macmillan Publishers Limited

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(A) Total protein (500 μg) from Tshr(+/+) and Tshr(−/−) mouse liver extracts were purified by IP with the HNF-4α antibody and subjected to WB with the p-Ser antibody. Total lysates were analyzed by WB with antibody against HNF-4α as indicated. Normal mouse IgG was used as a negative control. (B) Protein levels of cytoplasmic (-P) and nuclear (-N) HNF-4α were detected in Tshr(+/+) and Tshr(−/−) 8-week old littermate mice. All panels above are representative of 3 independent experiments. All the gels were run under the same experimental conditions, and key data cropped blots are used here. The full-length gel images are available in the Supplementary Figures.