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. 2015 Aug 25;5:11391. doi: 10.1038/srep11391

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Determination of changes in O₂^•- production and MDA and H₂O₂ content (A); the activities of SOD, POD, and CAT (B); plasma membrane (PM) H⁺-ATPase; and tonoplast H⁺-ATPase and H⁺-PPase in the mitochondria of cucumber roots at 3 d and 6 d after treatment. Samples were analyzed under normoxic conditions (Control), hypoxia treatment (Hypoxia) and hypoxia + 4 mM CaCl₂ treatment (Hypoxia + Ca²⁺). Values are means ± SE of three independent experiments. Bars marked with dissimilar letters are significantly different according to Duncan’s multiple range tests (P < 0.05).