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. 2015 Aug 25;5:63. doi: 10.3389/fcimb.2015.00063

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Copyright © 2015 Bermudez, Danelishvili, Babrack and Pham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The yeast two-hybrid interaction of MAV_2120, MAV_2235, and MAV_4563 proteins with the host target proteins. (a) COP9 signalosome subunit 5 (CSN5), (b) eukaryotic translation termination factor 1 (ETF1), (c) PNN-interacting serine/arginine-rich protein (PNISR), (d) serine/arginine-rich splicing factor 5 (SRSF5), (e) SECIS binding protein 2-like (SECISBP2L), (f) ubiquitin specific peptidase 48 (USP48). (B) Co-immunoprecipitation of MAV_2120 and MAV_4563 from M. smegmatis cell lysates using recombinant 6xHN:CSN5 and 6xHN:USP48 proteins. Bound proteins were captured on His-columns and visualized by western blotting using 6xHN and Flag antibodies.