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. 2015 Aug 25;5:13524. doi: 10.1038/srep13524

Figure 1. Simvastatin increases soluble epoxide hydrolase (sEH) phosphatase activity.

Figure 1

Phosphatase activity (PT) assay of bovine aortic endothelial cells (BAECs) (a) transfected with vector, full-length sEH (WT) or phosphatase domain of sEH (N-ter) for 48 h, then treated with or without an sEH phosphatase inhibitor, N-acetyl-S-farnesyl-L-cysteine (AFC, 0, 12.5, 25, 50, 100 μM) or ebselen (0, 2.5, 5, 10, 20 μM), for 2 h; (b) treated with vehicle or simvastatin (10 μM) for the indicated times; or (c) pretreated with AFC or ebselen (10 μM) for 2 h, then simvastatin (10 μM) for 30 min. Data are mean ± SEM from 5 independent experiments. *P < 0.05 vs. vehicle- or vector-treated cells, #P < 0.05 vs. simvastatin-treated alone cells.