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. 2015 Aug 25;5:13524. doi: 10.1038/srep13524

Figure 4. sEH phosphatase plays a crucial role in regulating simvastatin-induced Akt and AMPK phosphorylation.

Figure 4

Western blot analysis of (a) phosphorylated Akt (p-Akt) at Ser473 and Akt and (b) phosphorylated AMP-activated protein kinase (p-AMPK) at Thr172 and AMPK in BAECs pretreated with vehicle or AFC (50 μM) or transfected with vector, PTmut or N-ter for 48 h, then simvastatin (10 μM) for 0–120 min. Data are mean ± SEM from 5 independent experiments. *P < 0.05 vs. vehicle- or vector-treated cells, #P < 0.05 vs. simvastatin-treated alone cells.