Skip to main content
. 2015 Aug 25;5:13524. doi: 10.1038/srep13524

Figure 5. Simvastatin promotes the formation of an sEH–eNOS–Akt–AMPK complex.

Figure 5

BAECs were treated with simvastatin (10 μM) for 0–120 min. Cellular lysates was immunoprecipitated (IP) with anti-sEH Ab and then immunoblotted (IB) with (a) anti-eNOS, (b) anti-Akt, (c) anti-AMPK or anti-sEH Abs. Secreted human alkaline phosphatase (SEAP) activity in culture media from BAECs co-transfected with pM-sEH and (d) pVP-eNOS, (e) pVP-Akt or (f) pVP-AMPK for 48 h, then indicated concentrations of simvaststin (2.5, 5, 10 μM) for another 24 h. pM3-VP16–transfected cells were a positive control. Data are mean ± SEM from 5 independent experiments. *P < 0.05 vs. vehicle-treated group.