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. 2015 Aug 25;5:13524. doi: 10.1038/srep13524

Figure 8. c-Src signaling is essential for simvastatin-induced sEH tyrosine phosphorylation and the formation of an sEH–Akt–AMPK–eNOS complex.

Figure 8

BAECs were pretreated with or without PP1 (10 μM) for 2 h (a,c,e,g) or transfected with K298M for 48 h (b,d,f,h), then incubated with simvastatin (10 μM) for 15 min (a,b,e–g) or 30 min (c,d). Cellular lysates were IP with anti-sEH Ab and then IB with Ab for anti-sEH, (a,b) anti-Tyr phosphorylation, (c,d) eNOS, (e,f) Akt or (g,h) AMPK. Data are mean ± SEM from 5 independent experiments. *P < 0.05 vs. vehicle- or vector-treated group, #P < 0.05 vs. simvastatin-treated alone group.