Figure 3. Rimonabant modulates phospho-SFK^Tyr416 and RAF1 following systemic LPS injection.

Following hemodynamic measurements, lungs isolated from rats exposed to systemic LPS (5mg/kg) for 30 minutes with or without intracerebroventricular rimonabant pretreatment (500 ng in 0.5 μl saline + 2.5% DSMO), were separated into left and right halves. Right lungs were homogenized, and supernatants, 20 μg/lane, were Western blotted. A) Representative blots are shown for phospho-SFK^{Tyr 416}, total Src, RAF1, and β-actin. C=control, R=rimonabant, L=LPS and RL=rimonabant pretreatment + LPS. B) The Western blot band densities of phospho-Src^{Tyr 416} in relative density units (RDU). C) The Western blot band densities of Raf1 in relative density units (RDU). Data are mean ± S.E.M. Significance was at P<0.05. * = significantly different from control. # = significantly different from the LPS-only group. $ = significantly different from the ICV rimonabant + LPS group.