MVD staining in osteosarcoma patients and controls. CD34 staining was performed for MVD in tissues from osteosarcoma patients and osteochondroma control patients. The results showed that there were very low MVD staining in tissues from osteochondroma control patients (A, C), but significant higher MVD staining in tissues from osteosarcoma patients were observed (B, D, black arrows). (A, B, magnification 400×, C, D, magnification 100×).