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. 2015 Jul 15;7(7):1260–1270.

Figure 1.

Figure 1

Cloning and sequence alignment of the Der f 27 gene. A. Cloning and PCR of Der f 27 cDNA (M; DNA Marker 1; Der f 27 gene). B. Restriction enzymatic digestion results of recombinant Hyastatin-pET-32a (+) plasmid (M: DNA Marker; 1: Der f 27 DNA). C. The amino acid sequences of Der f 27. D. The phylogenetic tree analysis; Der f (Dermatophagoidesfarinae); Sars (Sarcoptesscabiei type hominis); Rhim (Rhipicephalusmicroplus); Ped p (Pedosphaeraparvula); Coll (Columba livia); Rhi h (Rhipicephalushaemaphysaloides); Ixos (Ixodesscapularis); Tact (Tachypleustridentatus); Stem (Stegodyphusmimosarum); Strc (Struthiocamelusaustralis); Nesn (Nestor notabilis); Corc (Corvuscornixcornix).