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Immunological characterization of Der f 27. A. Immunobloting analysis of specific IgE reactivity to allergen r-Der f 27 in the serum from r-Der f 27 SPT positive individuals. Lanes 1-3 indicatethe IgE positive serum from allergic subjects. Lane 4 indicatescontrol serum from healthy subjects using as negative controls. B. The specific IgE reactivity to allergen r-Der f 27 by ELISA. P1-P3, the serum from r-Der f 27 positive patients; C1 and C2, the serum from healthy control subjects.
