Figure 4.

Establishment of a mouse model of allergic asthma by r-Der f 27. A. The protocol for a mouse model of r-Der f 27-induce allergic asthma. B. Airway hyper-responsiveness were measured using the unrestrained whole-body plethysmography. The Penh values were recorded when the mice were stimulated with the increasing concentration of methacholine after the challenge. C and D. The level of IL-4 and INF-γ in BLAF were detected by ELISA. Samples of BLAF were collected on day 22, and levels of IL-4 and INF-γ were measured by ELISA. E. The number of eosinophil cells was counted in the BLAF. F-H. The pathologic changes of pulmonary tissue. F: The PBS group. G: The r-Der f 27 group. H: The DME group.