Figure 4.

Western blotting analysis of proteins linked to anthracycline-induced oxidative stress and cytoskeletal proteins. A. Representative immunoblots of total protein level of endothelial NO-synthase (eNOS), inducible NO-synthase (iNOS), heat shock protein 90 (hsp90), caveolin 3 and 1 (cav-3, cav-1), glycosylated subunit of the NADPH oxidase (gp91^phox) and manganese superoxide dismutase (MnSOD) from control (CON) and daunorubicin (DAU) group. B. Representative immunoblots of total protein level of cytoskeletal proteins α-tubulin, α-actinin and β-actin from CON and DAU. C. The concentration of thiobarbituric acid reactive substances (TBARSs) in ventricular samples. Data are shown as mean ± SEM, n = 6 per group.