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The expression of AHCY is determined by c-MYC activation in human breast cancer cell lines. A. c-MYC mRNA level by quantitative RT-PCR in human breast cancer cell lines. B. AHCY and c-MYC protein levels in breast cancer cells were analyzed by western blot and 30 µg proteins were loaded per well. C, D. 25 nM control, c-MYC and AHCY siRNA were treated in both MCF7 and MCF7-ADR cells for 48 h and the 30 µg protein levels were loaded per well and analyzed by western blot.
