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Regulation of MEK/ERK signaling in AHCY-knockdown breast cancer cells. A, C. Expression levels of Ras, C-/B-Raf, MEK, and ERK pathway molecules were detected by western blotting analysis in both MCF7 and MCF7-ADR cells and 30 µg of the proteins were loaded per well. B, D. Protein levels were quantified by densitometry using Multi gauge. The graphs show mean ± SD of three independent experiments. *means P<0.05 and **means P<0.01.
