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. 2015 Aug 25;5:13317. doi: 10.1038/srep13317

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Copyright © 2015, Macmillan Publishers Limited

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(a) Heatmap representation of ST6GAL1 transcript expression, as well as other glycosyltransferases, measured by global gene expression analysis in hPSCs and non-pluripotent cell samples. The expression of ST6GAL1 in each cell sample, detected by two independent probes targeting different regions of the ST6GAL1 transcript is shown. Black asterisk: WA07 hESCs and their isogenic differentiated derivatives. Purple asterisk: HDF51 cells and hiPSCs generated from HDF51 cells. (b) Upper panel: Western blotting analysis of ST6GAL1 in cell samples showed that undifferentiated hPSCs had higher ST6GAL1 protein expression, compared to differentiated cells. Lane 1: HMi-506_Mel Diff¹⁶, 2: HDF68i-505_Mel Diff¹⁶, 3: HM (HEMl), 4: HDF68, 5: HDF51, 6: WA09, 7: HDF68i-505¹⁶, 8: HMi-506¹⁶, 9: TSRI001i-HDF⁸. Lower panel: SNA-mediated blotting showed that protein samples extracted from undifferentiated hPSCs had higher reactivity to SNA binding. Lane 1: HM, 2: HDF68i-505_Mel Diff, 3: HDF51, 4: HDF68, 5: WA09, 6: HMi-506, 7: TSRI001i-HDF, 8: HDF68i-505. (c) SNA-mediated blotting showed that protein samples extracted from derivatives of undirected differentiation (embryoid bodies, EBs) had less reactivity to SNA, compared to those from paired, undifferentiated hPSCs. Lane 1: WA09, 2: WA07, 3: TSRI001i-HDF, 4: HMi-506, 5: WA09_EBs, 6: WA07_EBs, 7: TSRI001i-HDF_EBs, 8: HMi-506_EBs. (d) Flow cytometric analysis demonstrated that live human cells in the pluripotent state were more reactive to SNA at the cellular level.