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. 2015 Feb 26;212(6):934–938. doi: 10.1093/infdis/jiv124

Figure 1.

Figure 1.

SaeR/S alters cell fate and suppresses IL-8 production in neutrophils. PMNs were subjected to USA300 or ΔsaeR/S at low MOI. Percent PMNs with condensed chromatin assessed via FACS at designated times (A). IL-8 release from PMNs assessed by ELISA (B) and intracellular IL-8 measured in PMNs at 6 hours postinfection via FACS (C). Whole blood was treated with USA300 or ΔsaeR/S and percent NF-κB-p65(P)+ cells from CD11bHi+ population was quantified by FACS at designated time points (D). Data shown are the means ± SEM of 4–10 experiments (A) or 3–4 experiments (B–D). *P < .05 determined by 1-way ANOVA with Tukey's posttest (A, C, and D) comparing USA300 to ΔsaeR/S groups or paired t test (B). Abbreviations: ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; FACS, fluorescence-activated cell sorter; IL-8, interleukin-8; MOI, multiplicity of infection; nuclear factor-κB, NF-κB; NF-κB-p65(P), phosphorylated NF-κB p65 subunit; PMNs, polymorphonuclear leukocytes; SEM, standard error of the mean.