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. 2015 Feb 26;212(6):934–938. doi: 10.1093/infdis/jiv124

Figure 2.

Figure 2.

IL-8 promotes neutrophil survival and bacterial clearance. PMNs treated with 20 µM parthenolide or DMSO control were infected with USA300 or ΔsaeR/S at low MOI for 6 hours followed by analysis of PMNs with condensed chromatin (A) and bacterial burden (B). In C–D, PMNs were exposed to RIL-8 (25 ng/mL) and infected with USA300 or ΔsaeR/S for 6 hours followed by FACS analysis of condensed chromatin (C) or assessment of USA300 survival. D, Data shown are the means ± SEM of 3–5 separate experiments. *P < .05 determined by 1-way ANOVA with Tukey's posttest (A–C) or by paired t test (D) comparing USA300 to ΔsaeR/S groups. Abbreviations: ANOVA, analysis of variance; CFU, colony-forming unit; DMSO, dimethyl sulfoxide; FACS, fluorescence-activated cell sorter; IL-8, interleukin-8; MOI, multiplicity of infection; NS, not significant; PMNs, polymorphonuclear leukocytes; RIL-8, recombinant IL-8; SEM, standard error of the mean.