Figure 6.
Decrease in anti hepatitis C virus (HCV) neutralizing antibody breadth after incident human immunodeficiency virus (HIV) infection occurs in subjects with CD4+ T-cell loss. A–C, The decrease in neutralizing breadth after incident HIV infection was not significant in subjects with CD4+ T-cell counts ≥350/mm3, but breadth declined significantly in subjects with counts <200/mm3. Grey lines represent number of HCVpp neutralized (neutralizing breadth) for individual subjects measured at 2 time points; black lines, medians (overlapping lines are dithered for clarity). Positive neutralization of each of the HCVpp was noted when neutralization was >25%, which was >2 standard deviations above the mean neutralization of negative control murine leukemia virus pseudoparticles by all 25 serum samples tested. Wilcoxon signed rank test was used to calculate significance of the change in number of HCVpp neutralized; when normality was satisfied, paired t tests were used. D, Comparison of the change in the number of HCVpp neutralized over time in HCV-monoinfected controls and HIV/HCV-coinfected subjects stratified by post-HIV CD4+ T-cell count. Symbols represent changes for individual subjects; black lines, medians. Rank sum tests were used to calculate significance; normality was satisfied, t tests were used.