Table 1.
Comparison of MOI and Diversity by Ion Torrent Amplicon Deep Sequencing Versus Microsatellite Genotyping (N = 65 Samples)
| Deep Sequencing | Microsatellite Genotyping |
||||
|---|---|---|---|---|---|
| Pvmsp1 | PvMS7 | PvMS10 | MS10.13 | MS Markers Combined | |
| Median MOI | 3 | 1 | 1 | 2 | 2 |
| Mean MOI | 3.8 | 1.5 | 1.4 | 1.8 | 2.1 |
| Max MOI | 10 | 3 | 4 | 5 | 5 |
| No. polyclonal | 60 | 27 | 17 | 33 | 47 |
| % polyclonal | 92% | 42% | 26% | 51% | 72% |
| No. alleles | 52 | 19 | 15 | 20 | NA |
| Virtual heterozygosity (HE) | 0.94 | 0.92 | 0.84 | 0.94 | NA |
Abbreviations: MOI, multiplicity of infection; MS, microsatellite; NA, not applicable.