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. 1988 Nov;7(11):3531–3537. doi: 10.1002/j.1460-2075.1988.tb03229.x

Two guanosine binding sites exist in group I self-splicing IVS RNAs.

P S Kay 1, P Menzel 1, T Inoue 1
PMCID: PMC454854  PMID: 3208741

Abstract

A shortened form of the self-splicing rRNA intervening sequence (IVS) of Tetrahymena thermophila can catalyze a transesterification reaction, termed G-exchange, between a monomeric guanosine derivative such as GTP and the substrate GpN (where N is A, C, G or U). The reaction is specific to the two guanosines involved, providing evidence that two guanosine binding sites exist in this group I IVS RNA. One binding site accommodates a guanosine which initiates self-splicing and the other recognizes the guanosine preceding the 3' splice site. Previously, only one guanosine binding site was thought to be involved in the mechanism of self-splicing. Based on the two functionally distinguishable guanosine binding sites, a new model is proposed to explain how the two independent transesterification reactions required for self-splicing might proceed in a concerted manner.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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