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. 2015 Aug 26;35(9):1987–1994. doi: 10.1161/ATVBAHA.115.305969

Figure 2.

Figure 2.

Exogenous arachidonic acid (AA) evokes Orai3-dependent Ca2+ entry and microsomal glutathione S-transferase 2 (MGST2) is required in the action of vascular endothelial growth factor (VEGF). A, Example responses and mean data of human umbilical vein endothelial cells (HUVECs) exposed to exogenous AA (40 μmol/L). All cells were pretreated with thapsigargin (2 μmol/L) and studied in the presence of S66 (5 μmol/L). Cells were transfected with scrambled (scr.) or Orai3 (O3_1) siRNA; (n=3/N=60 each). B, Representative immunoblot of MGST2 in HUVECs transfected with scr. or MGST2 siRNA_1 or MGST2 siRNA_2. Untransfected Huh7 cells were used as a positive control for MGST2 expression. C, Example VEGF responses and mean data from HUVECs transfected with scr., O3_1 or MGST2_1 siRNA. Mean data are for VEGF responses at peak or after 330 s (n=3/N=36 each). Data are represented as mean±SEM; *P<0.05; ns P>0.05.