Figure 5.

Analysis of MGST2, LTC4S, and Orai3 gene expression in human umbilical vein endothelial cell (HUVEC). A, Example agarose gel for end-point polymerase chain reaction products obtained with primers for MGST2, LTC4S, Orai3, and β-actin from HUVEC cDNA. Cells were exposed to static and shear-stress conditions, respectively, before harvesting mRNA and reverse transcriptase reaction (+RT [reverse transcriptase]) to generate cDNA. −RT denotes control reaction. B, Mean data and analysis of MGST2 and Orai3 mRNA (n=6). Data are represented as mean±SEM; *P<0.05.