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. 2015 Aug 25;8:435. doi: 10.1186/s13071-015-1039-3

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© Costales et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — LSSP-PCR. DNA from parafinized heart explants and blood samples was amplified using the S35/S36 primer pair. The 330 bp product was gel-purified and analyzed by LSSP-PCR. Products were run in a 10 % polyacrilamide gel and silver-stained. H1 and H2 correspond to DNA extracted from two different sections of parafinized heart explant tissue B and B2 correspond to blood samples taken 10 days apart, starting six weeks after transplant. Cl3 corresponds to DNA from one of the clones derived from the original T. cruziisolate. Y indicates “Y strain” parasites (TcII DTU) used for comparison. MWM = molecular weight markers