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. 2015 Aug 25;14:330. doi: 10.1186/s12936-015-0846-5

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© de Souza et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Detection of multiple-clone infections in field samples. The six markers were genotyped in 51 samples from malaria-endemic area of Brazil. A cut-off value of one-fourth was considered for the analysis. a The frequency of multi-clonal infections was calculated considering both criteria for minor allele detection: a cut-off value of one-third (black bars only) or one-fourth (the entire bar, including both black and grey bars) of the height of the predominant peak. The increase in the rate of detection of multiple-clone infections with the one-fourth criterion is highlighted in grey. b Results of genotyping are shown for the 36 samples with multiple-clone infection detected by one (hatched rectangles) or more markers (rectangles colored in black). Each column represents the same sample genotyped with the six markers. The frequency of multi-clonal infections (% Total) was calculated for each marker. The increase in the number of multiple-clone infections detected, resulting from the combination of two or more markers, is indicated as the cumulative percentage (% Cum)