Fig. 1.

PLIN family members in control and CETP-deficient (CETP def) cells. Cells were incubated with or without 200 μM unlabeled oleate/BSA for 48 h. A: PLIN mRNA levels were measured by qPCR. Values are the average of two experiments and representative of two additional experiments comparing wild-type SW872 cells with CETP-deficient cells. *P < 0.05 versus control without oleate; ⁺P < 0.05 versus control with oleate; ^#P < 0.05 versus CETP-deficient cells incubated without oleate. PLIN1 (perilipin); PLIN2 (adipose differentiation-related protein); PLIN3 (TIP47). B: Western blot of PLINs in the 100,000 g supernatant of cells incubated with oleate/BSA as above. The positions of molecular mass standards, in kDa units, are shown. Results are typical of three separate studies.