TABLE 2.
TG and DG content of ER
| Cell Type | TG (cpm/μg protein) | DG (cpm/μg protein) | TG (cpm/nmol phospholipid) | DG (cpm/nmol phospholipid) |
| Control | 7.69 ± 0.93 | 1.49 ± 0.12 | 5.39 ± 0.13 | 1.06 ± 0.17 |
| CETP-deficient | 6.90 ± 1.55 | 1.52 ± 0.73 | 4.71 ± 0.89 | 1.29 ± 0.43 |
Cells were incubated with 200 μM 3H-oleate/BSA for 48 h to label cellular lipid pools. Highly purified ER was isolated by gradient ultracentrifugation. Lipids were extracted and fractionated by TLC. Shown are the mean ± SD of values determined on three separate ER preparations.