TABLE 3.
TG hydrolase activity in microsomes
| Cell Type | Without Oleate | With Oleate |
| Control | 44.2 ± 1.6 | 17.3 ± 5.4a |
| CETP-deficient | 48.9 ± 0.2 | 37.5 ± 2.8a,b |
Control and CETP-deficient cells were grown to near confluence then treated with or without 200 µM oleate/BSA for 48 h. Microsomes were isolated and TG lipolytic activity was measured with micelles containing 3H-TG. Values are picomoles of TG hydrolyzed per 200 μg protein per 4.5 h. Data are the mean ± SD (n = 2) and are representative of two experiments on separate microsome preparations.
a
P < 0.05 versus same cell without oleate.
b
P < 0.05 versus control with oleate.