Fig. 5.

Capacity for cold-stimulated TG lipolysis in WAT. Pemt^+/+ and Pemt^−/− mice (8–9 weeks old) were fed the HF diet for 2 weeks followed by a 4 h fast and cold exposure. A: Plasma levels of epinephrine (E) and norepinephrine (NE). B: Representative immunoblots of lipolytic enzymes: phospho-HSL (p-HSL), total-HSL (HSL), ATGL, TGH, and their densitometric analysis relative to the loading control GAPDH. C: Levels of plasma NEFAs. All data are from five to seven mice of each group; *P < 0.05 by Student t-test for Pemt^+/+versus Pemt^−/−.