TABLE 2.
Flows and solvent composition for the LC method
| Step | Seconds | Flow (ml/min) | Solvent A (%) | Solvent B (%) |
| 0 | 0 | 0.3 | 80 | 20 |
| 1 | 390 | 0.3 | 66 | 34 |
| 2 | 200 | 0.3 | 1 | 99 |
| 3 | 60 | 0.4 | 1 | 99 |
| 4 | 60 | 0.3 | 80 | 20 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.