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. Author manuscript; available in PMC: 2015 Aug 25.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jan 18;319(0):129–142. doi: 10.1016/j.mce.2010.01.019

Figure 7. Proteasome inhibition rapidly increases BRCA1 protein levels.

Figure 7

(A) MCF7 cells were cultured in CSS medium for 48 hours prior to treatment with 20 μM MG132 alone or in combination with 10 μM LY294002 for the indicated amount of time (in hours). Control cells were treated with EtOH vehicle (V) alone. Cell lysates were immunoblotted with the indicated antibodies. Tubulin is included as an additional loading control.

(B) Similarly cultured MCF7 cells were treated with the clinically utilized proteasome inhibitor bortezomib at either 0.1 μM or 1 μM for the indicated times prior to harvest for immunoblot analysis.