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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Aug 24;35(18):2299–2310. doi: 10.1038/onc.2015.299

Figure 5.

Figure 5

NF-κB1 p105 stabilizes Tpl2 in lung cancer cells. (a) Real-time PCR showing a similar expression of Tpl2 mRNA in NF-κB1Δ/Δ tumors and WT tumors from urethane-treated mice. Data shown are means ± SD (n ≥ 3). (b) IHC staining showing no Tpl2 protein in NF-κB1Δ/Δ tumors from urethane-treated mice. Scale bar: 20 µm. (c) IB assays showing the protein expression levels of p105, p50 and Tpl2 in human lung cancer cell lines. (d) Quantitation of IB assays in C showing a positive correlation in the protein expression levels of NF-κB1 and Tpl2 in human lung cancer cell lines. (e) IB assays showing restoration of Tpl2 protein expression in human lung cancer cell lines stably expressing HA-p105, but not HA-p50.