Figure 2.

Polymerization blocking activity of the 4B12 monoclonal antibody. A) Nondenaturing PAGE followed by silver staining of 0.02 mg/ml monomeric Z α₁-antitrypsin incubated with serial 1:2 dilutions of purified mAb4B12 starting from 1:1 molar ratio (0.063 mg/ml) at 45°C for 60 hours. Right) ELISA quantification of α₁-antitrypsin polymers by sandwich ELISA (2C1-Ag-9C5-HRP) as means ± sem, n = 5, each performed in triplicate. B) Quantification of α₁-antitrypsin polymers after incubation with purified Fab domain of mAb4B12 in the same conditions as A as means ± sem; n = 5, each performed in triplicate. C) Nondenaturing PAGE followed by silver staining of samples processed as in A but in the presence of nonspecific IgG₁. Right) ELISA quantification for α₁-antitrypsin polymers (n = 3). m, monomer; p, polymers.