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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Cancer Res. 2015 May 4;75(13):2699–2707. doi: 10.1158/0008-5472.CAN-15-0400

Figure 1. Spatiotemporal recruitment kinetics of Brca1 and other DDR factors to the DSB.

Figure 1

ChIP assay showing the recruitment of (A) Brca1, (B) Nbs1, (C) γH2AX, (D) MDC1, (E) RNF8 and (F) RAP80 to the DSB induced by I-PpoI on chromosome 1 in MCF7 cells expressing ddI-PpoI that were cultivated in medium containing 0.1% fetal bovine serum for 24 h prior to DSB induction. Time indicated is hours following addition of 4-OHT. The I-PpoI cleavage site on chromosome 1 is located at distance 0. Data of two independent experiments are shown as mean +/− SEM. The y-axis displays the fold change in relative occupancy normalized to the control.