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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Cancer Res. 2015 May 4;75(13):2699–2707. doi: 10.1158/0008-5472.CAN-15-0400

Figure 5. RAP80-dependent recruitment of Brca1 to the DSB surrounding regions is required for Brca1 phosphorylation by ATM.

Figure 5

Immunoblot showing the ATM-specific phosphorylation of Brca1 at serine 1387 and 1423 and the activation of the ATM pathway in (A) MCF7 and (B) HeLa cells 30 min after treatment with 10 Gy IR. Cells were transfected with either non-targeting control siRNA, RAP80, NBS1 or ATM targeting siRNA. (←) ATM band, (*) Non-specific band.