Table 1. Probe specifications.
| Name | Target | Probe sequence (5’-3’) | Tm (°C) | %GC | Reference |
|---|---|---|---|---|---|
| AtoITM1 | A. vaginae | Alexa488-OO-CTC-CTG-ACC-TAA-CAG-ACC | 66 | 55.6 | Newly designed, based on Burton et al. [28] |
| AtoITM2 | A. vaginae | Alexa488-OO-GCG-GTY-TGT-TAG-GTC-AGG | 72 | 58.3 | Newly designed, based on Fredricks et al. [29] |
| Ato291 | A. vaginae | Alexa488-OO-GGT-CGG-TCT-CTC-AAC-CC | 68 | 60.0 | Newly designed, based on Harmsen et al. [27] |
| Gard162 | G. vaginalis | Alexa647-OO-CAG-CAT-TAC-CAC-CCG | 61 | 60.0 | Machado et al. [30] |
| BacUni-1 | Eubacteria | Alexa555-CTG-CCT-CCC-GTA-GGA | 64 | 66.7 | Perry-O'Keefe et al. [32] |