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. 2015 Mar 26;19(8):1795–1804. doi: 10.1111/jcmm.12583

Figure 1.

Figure 1

(A) Cell growth analysis of PC-3, DU-145 and LNCaP cells. Tumour cells were treated with either 100 U/ml IFNα, 1 nM everolimus or 1 mM VPA, or with all compounds simultaneously (TD). Controls remained untreated. Cells were counted after 24, 48 and 72 hrs. One representative experiment of six is shown. * indicates significant difference to controls, # indicates significant difference to single drug treatment. (B) Adhesion of prostate cancer cells to HUVEC. PC-3, DU-145 and LNCaP cells were treated with 100 U/ml IFNα, 1 nM everolimus or 1 mM VPA, or with all compounds simultaneously (TD). Controls remained untreated. Tumour cells were added at a density of 0.5 × 106 cells/well to HUVEC monolayers for 1, 2 or 4 hrs. Non-adherent tumour cells were washed off and the remaining cells fixed and counted in five different fields (5 × 0.25 mm2). Mean values were calculated from five counts. One representative of six experiments is shown. * indicates significant difference to controls, # indicates significant difference to single drug treatment.