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. 2015 Mar 17;19(8):1836–1846. doi: 10.1111/jcmm.12555

Figure 4.

Figure 4

INS-1E cell aggregates of various sizes remain viable and functional up to 7 days of culture. (A) INS-1E cell aggregates were formed using agarose microwells. After 7 days of culture, a viability assay was performed to visualize living cells (green) and dead cells (red). (B) Histological evaluation of INS-1E cell aggregates of 50, 100 and 500 cells after 7 days of culture. Both DAB chromogen and fluorescent anti-insulin staining demonstrate that INS-1E cell aggregates of various sizes express insulin; scale bars = 50 μm. (C) Glucose-stimulated insulin secretion of INS-1E cell aggregates of various sizes measured after 7 days of culture (100 aggregates per condition, in triplicate); error bars represent ±SD.