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. 2015 Mar 6;19(8):1857–1867. doi: 10.1111/jcmm.12557

Figure 2.

Figure 2

SIRT1 regulates ET-1 and TGF-β1 via p300. HG caused increased (A) p300 mRNA and reduced (B) SIRT1 mRNA levels in ECs. (A) Ad-SIRT1 transfection in HG prevented p300 up-regulation and SIRT1 knockdown with siRNA in NG up-regulated p300 mRNA levels. (B) p300 siRNA transfection in HG (efficiency in A) normalized SIRT1 expression. Furthermore, p300 overexpression in NG (efficiency in A) reduced SIRT1 mRNA levels. (C and D) HG induced ET-1 and TGF-β1 up-regulation was abrogated with p300 siRNA (not with scramble) transfection. p300 overexpression in NG up-regulated the expressions of these transcripts. (E and F) Rescue experiment showing up-regulation of ET-1 and TGF-β1 mRNA levels in NG with SIRT1 knockdown can be resolved with subsequent p300 knockdown in the ECs. NG = 5 mmol/l glucose, HG = 25 mmol/l glucose, OSM = osmotic control, * = significantly different from NG, ╪ = significantly different from HG, mRNA levels are expressed as a ratio of 18s. All data (mean ± SEM, P < 0.05) were normalized to NG; n = 4–6/group.