Skip to main content
. 2015 Mar 5;19(8):1877–1886. doi: 10.1111/jcmm.12559

Figure 3.

Figure 3

The role of S6, P70S6K and mTOR in the regulatory mechanism of AKT and ERK in morroniside-treated OA chondrocytes. (A) Cells were pre-treated with LY294002 (25 μM) for 2 hrs prior to treatment with morroniside (0.1 μM) for 24 hrs. The level of p-S6 was then detected by western blotting analysis. (B) Cells were pre-treated with U0126 (100 μM) for 2 hrs prior to treatment with morroniside (100 μM) for 24 hrs, and the levels of p-P70S6K and p-S6 were detected by western blotting analysis. The values represent the mean ± SEM of three to five independent experiments, each yielding similar results (*P < 0.05).