Figure 1.

Pluripotency of iPSC derived from CPVT1 RyR2^R420Q and CPVT2 CASQ2^D307H patients and healthy control. (A–C) Immunostaining of typical pluripotent markers shown for iPSCs derived from (A) healthy control clone HDF24.2, (B) CPVT1 patient, clone HDF15.4 and (C) CPVT2 patient, clone 20.1. Nuclei were stained with DAPI (blue), scale bar 50 μm. (D–F) Karyotype analysis of iPSC derived from (D) healthy control clone HDF24.2, (E) CPVT1 patient clone HDF15.4 and (F) CPVT2 patient clone 20.1. (G–I) Histological analysis of a representative teratoma obtained from in vivo differentiated cells of (G) control iPSC-24S9, (H) CPVT1 iPSC-15.4 and (I) CPVT2 iPSC-20.1. The formed teratomas contained derivatives of all three germ layers (ectoderm, mesoderm and endoderm). Mesoderm: (m) muscle, (c) cartilage, (b) blood cells. Endoderm: (e) epithelium, (g) endocrine glands. Ectoderm: (n) neural rosette. All images were obtained from formalin-fixed and paraffin-embedded teratoma sections stained with haematoxylin and eosin, scale bar 50 μm.