Figure 3.

Cardiac differentiation of iPSC-derived from CPVT1 RyR2^R420Q and CPVT2 CASQ2D307H patients and healthy control. (A) Immunofluorescence expression of typical myofilament in cardiomyocytes (62–64 days old) of control (clone KTN3), CPVT1 (clone 15.4) and CPVT2 (clone 19.1) stained for the typical myofilament proteins; α-actinin and cardiac troponin, scale bar 10 μm. (B) Spontaneous beating rate of control-CM (n = 9), CPVT1-CM (n = 10) and CPVT2-CM (n = 10), bpm: beats per minute. Statistical analysis of one-way anova followed by Dunn’s test, *P < 0.05.